N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis (major enzyme)

N-acetylglucosamine-malate deacetylase, involved in bacillithiol synthesis (major enzyme)

BSU22470

BSU_22470

224

236

672

711

N-acetylglucosamin-malate deacetylase

N-acetylglucosamine-malate deacetylase

BSU22470

BSU_22470

2,358,208 → 2,358,918

2,358,208 2,358,918

GlcNAc-Mal → GlcN-Mal + Ac

(S)-malyl N-acetyl-α-D-glucosaminide + H₂O --> (S)-malyl α-D-glucosaminide + acetate (according to UniProt)

[[protein|BshB2]]

[[this]]

[PDB|2IXD] (from B. cereus, 62% identity)

[PDB|6ULL] (complex with substrate analogue) [pubmed|31867856]

[PDB|6P2T] (complex with citrate) [pubmed|31867856]

[PDB|2IXD] (from B. cereus, 62% identity) [pubmed|17501983]

MGNA-A409 (ypjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/409 NBRP B. subtilis, Japan]

''bshB1'' null mutant and ''[[gene|bshB1]]'' / ''[[gene|bshB2]]'' double null mutant available in [SW|John Helmann] lab

GP87 (''ypjG''::''pX2''(''cat'')), available in [SW|Stülke] lab

MGNA-A409 (ypjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/409 NBRP B. subtilis, Japan]

''bshB1'' null mutant and ''[[gene|bshB1]]'' / ''[[gene|bshB2]]'' double null mutant available in [SW|John Helmann] lab

GP87 (''ypjG''::''pX2''(''cat'')), available in [SW|Jörg Stülke]'s lab

BKE22470 ([[gene|bshB1]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG

BKK22470 ([[gene|bshB1]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG

20308541, 19578333, 23894131, 23758290, 25988368

20308541, 19578333, 23894131, 23758290, 25988368, 17501983, 31867856

PIGL family (with [[protein|BshB2]], according to UniProt)

Zn²⁺ [pubmed|31867856]